An Electrochemical DNA-Hybridization Assay for Acinetobacter baumannii Detection

作者：Wang, Yanping; He, Haiwu; Liu, Haizhen; Feng, Cui; Yao, Zhongji^*

来源：International Journal of Electrochemical Science, 2018, 13(1): 1051-1061.

DOI：10.20964/2018.01.72

• DNA
• Acinetobacter baumannii
• Gold electrode
• beta-cyclodextrin
• Differential pulse voltammetry

The present paper reported the analysis of the Acinetobacter baumannii using an electrochemical biosensor fabricated based on a gold electrode (AuE) with the electroactive label of beta-cyclodextrin (beta-CD). Due to the formation of an Au-S bond, a thiol decorated single-stranded DNA probe was covalently immobilized onto the electrode surface. For the working mechanism of differential pulse voltammetry (DPV) on the DNA hybridization, the electrochemical signals of the beta-CD binding reduction to the double-stranded DNA (ds-DNA) were recorded. Moreover, the as-prepared biosensor has the potential of being used for the analysis of Acinetobacter baumannii in excrement specimens.

• 出版日期2018-1
• 单位海南医学院