科研之友
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摘要
Transgenic poultry with a gene of human granulocyte colony-stimulating factor (gcsf) was developed by artificial insemination with the transfected sperm using the LipofectaminA (R) 2000 reagent. The ratio of transgenic chickens carrying the human gcsf genome was 33.3% of the total number of birds obtained. It was shown that the concentration of the human G-csf protein in blood serum of transgenic specimens varied from 50 to 220 pg/mL. The first generation of poultry was obtained as a result of insemination of transgenic hens by sperm of anti-transgenic cocks, thereby inheritance of the foreign gene was found in 37.5% of the obtained chickens.
- 出版日期2013-9
