A comparative analysis of total serum miRNA profiles identifies novel signature that is highly indicative of metastatic melanoma: a pilot study

作者：Greenberg Eyal; Besser Michal J; Ben Ami Eytan; Shapira Frommer Ronnie; Itzhaki Orit; Zikich Dragoslav; Levy Daphna; Kubi Adva; Eyal Eran; Onn Amir; Cohen Yehudit; Barshack Iris; Schachter Jacob; Markel Gal^*

来源：Biomarkers, 2013, 18(6): 502-508.

DOI：10.3109/1354750X.2013.816777

• Biomarkers
• circulating microRNAs
• diagnostic
• melanoma
• serum

Context Quantification of circulating microRNAs (miRNAs) has recently become feasible and reliable, with most efforts focusing on miRNAs overexpressed by cancer cells.
Objective: Identification of a characteristic circulating miRNAs profile in melanoma patients.
Methods: We conducted a pilot study comprised of unbiased qPCR comparison of serum miRNA profiles between metastatic melanoma patients and healthy donors.
Results: Loss of two normal serum-miRNAs, miR-29c and miR-324-3p, is highly indicative of metastatic melanoma. Hierarchical clustering analysis supported the results and clearly distinguished melanoma patients from healthy donors, metastatic colon and renal cancer patients.
Discussion and conclusions: This approach is independent of tumor heterogeneity and is expected to have superior biomarker performances.

• 出版日期2013-9