Dereplication of cytotoxic compounds from different parts of Sophora pachycarpa using an integrated method of HPLC, LC-MS and H-1-NMR techniques

作者：Shour Sara; Iranshahy Milad^*; Ngoc Pham; Quinn Ronald J; Iranshahi Mehrdad^*

来源：Natural Product Research, 2017, 31(11): 1270-1276.

DOI：10.1080/14786419.2016.1239095

• Sophora pachycarpa
• alopecurone A
• flavonostilbene
• cytotoxicity

Sophora pachycarpa Schrenk ex C.A.Mey. is an annual plant belonging to the family Fabaceae. The cytotoxic activities of methanol-dichloromethane extracts (1:1) of different parts of S. pachycarpa were investigated on DU145 (prostate cancer cell line) and MCF-7 (breast cancer cell line) cell lines. The root extract of S. pachycarpa was the only extract that showed significant cytotoxic activity with IC50 values of 39.88 and 16.49g/mL on DU145 and MCF-7 cell lines, respectively. The root extract was then subjected to RP-HPLC for further fractionations. Among the isolated fractions from root extract, only one of them had remarkable cytotoxic effects with IC50 value of 26.43 on MCF-7 and 7.54g/mL on DU145 cell lines. Further purification led to isolation of a compound with IC50 values of 5.44 and 2.44g/mL on MCF-7 and DU145 cell lines, respectively. Based on H-1 NMR and C-13 NMR spectra, together with LC-MS, the structure of the purified compound was assigned as the flavonostilbene alopecurone A.
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• 出版日期2017