科研之友
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摘要
Mast cells play important roles via Fc epsilon RI-mediated activation in allergic asthma. A nonpolymorphic MHC I-like molecule CD1d, which is mainly expressed in APCs, presents glycolipid Ag to iTCR on iNKT cells and modulates allergic responses. This study aimed to investigate the role of CD1d on IgE production and mast cell activation related to allergic asthma. Bone marrow-derived mast cells (BMMCs) from C57BL/6 Wild type (WT) or KO (CD1d(-/-)) mice were activated with Ag/Ab (refer to WT-act-BMMCs and KO-act-BMMCs, respectively) or a-Galactosylceramide (WT-alpha Gal-BMMCs, KO-alpha Gal-BMMCs) in the presence of iNKT cells. WT, KO or BMM-Ctransferred KO mice were sensitized and/or challenged by OVA or et-Gal to induce asthma. KO-act-BMMCs reduced intracellular Ca2+ levels, expression of signaling molecules (Ras, Rac1/2, PLA(2), COX-2, NF-kappa B/AP-1), mediator release (histamines, leukotrienes and cytokines/chemokines), and total IgE levels versus the corresponding VVT-BMMCs. KO mice reduced total and OVA-specific serum IgE levels, number of mast cells, recruiting molecules (CCR2/CCL2, VCAM-1, PECAM-1), expression of tryptase, c-kit, CD40L and cytokine mRNA, colocalization of c-kit and CD1d or iNKT cells in BAL cells or lung tissues, and PCA responses, compared with the corresponding WT mice. BMMC-transferred KO-both mice showed the restoration of all allergic responses versus KO-both mice (Ag/Ab reaction plus a-Gal). KO-aGal-BMMCs or KO-alpha Gal mice did not show any responses. Our data suggest that CD1d-expressed mast cells may function as APC cells for iNKT cells and exacerbate airway inflammation and remodeling through up-regulating IgE production via B cell Ig class switching and mediator release in mast cells of OVA-challenged mice.
- 出版日期2014-5
