A conserved alpha beta transmembrane interface forms the core of a compact T-cell receptor-CD3 structure within the membrane

作者：Krshnan Logesvaran; Park Soohyung; Im Wonpil; Call Melissa J^*; Call Matthew E

来源：Proceedings of the National Academy of Sciences of the United States of America, 2016, 113(43): E6649-E6658.

DOI：10.1073/pnas.1611445113

• T-cell receptor
• transmembrane structure
• NMR
• MD simulation
• cysteine cross-linking

The T-cell antigen receptor (TCR) is an assembly of eight type I single-pass membrane proteins that occupies a central position in adaptive immunity. Many TCR-triggering models invoke an alteration in receptor complex structure as the initiating event, but both the precise subunit organization and the pathway by which ligand-induced alterations are transferred to the cytoplasmic signaling domains are unknown. Here, we show that the receptor complex transmembrane (TM) domains form an intimately associated eight-helix bundle organized by a specific interhelical TCR TM interface. The salient features of this core structure are absolutely conserved between alpha beta and gamma delta TCR sequences and throughout vertebrate evolution, and mutations at key interface residues caused defects in the formation of stable TCR alpha beta:CD3 delta epsilon: CD3 gamma epsilon:zeta zeta complexes. These findings demonstrate that the eight TCR-CD3 subunits form a compact and precisely organized structure within the membrane and provide a structural basis for further investigation of conformationally regulated models of transbilayer TCR signaling.

• 出版日期2016-10-25
• 单位河北医科大学