Natural Killer Cell-Mediated Host Defense against Uropathogenic E. coli Is Counteracted by Bacterial HemolysinA-Dependent Killing of NK Cells

作者：Gur Chamutal; Coppenhagen Glazer Shunit; Rosenberg Shilo; Yamin Rachel; Enk Jonatan; Glasner Ariella; Bar On Yotam; Fleissig Omer; Naor Ronit; Abed Jawad; Mevorach Dror; Granot Zvi; Bachrach Gilad^*; Mandelboim Ofer

来源：Cell Host & Microbe, 2013, 14(6): 664-674.

DOI：10.1016/j.chom.2013.11.004

Uropathogenic Escherichia coli (UPEC) are a common cause of urinary tract infections (UTIs) in humans. While the importance of natural killer (NK) cells in innate immune protection against tumors and viral infections is well documented, their role in defense against bacterial infections is still emerging, and their involvement in UPEC-mediated UTI is practically unknown. Using a systematic mutagenesis approach, we found that UPEC adheres to NK cells primarily via its type I fimbriae and employs its hemolysinA toxin to kill NK cells. In the absence of hemolysinA, NK cells directly respond to the bacteria and secrete the cytokine TNF-alpha, which results in decreased bacterial numbers in vitro and reduction of bacterial burden in the infected bladders. Thus, NK cells control UPEC via TNF-alpha production, which UPEC counteracts by hemolysinA-mediated killing of NK cells, representing a previously unrecognized host defense and microbial counterattack mechanism in the context of UTI.

• 出版日期2013-12-11