Anti-inflammatory and anti-genotoxic activity of branched chain amino acids (BCAA) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages

作者：Lee Jae Hoon; Park Eunju; Jin Hyue Ju; Lee Yunjeong; Choi Seung Jun; Lee Gyu Whan; Chang Pahn Shick; Paik Hyun Dong^*

来源：Food Science and Biotechnology, 2017, 26(5): 1371-1377.

DOI：10.1007/s10068-017-0165-4

• Branched-chain amino acid
• Anti-inflammatory activity
• Anti-genotoxic activity
• RAW 264.7 macrophage

The purpose of this study was to evaluate the anti-inflammatory and anti-genotoxic activity of branched-chain amino acids (BCAAs) in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. BCAAs inhibited LPS-induced NO production, with 100 mM leucine having the most pronounced effect, suppressing NO production by 81.15%. Valine and isoleucine also reduced NO production by 29.65 and 42.95%, respectively. Furthermore, BCAAs suppressed the inducible nitric oxide synthase mRNA expression. Additionally, BCAAs decreased the mRNA expression of interleukin-6 and cyclooxygenase-2 which are proinflammatory mediators. Anti-genotoxic activities of BCAAs were assessed using the alkaline comet assay and valine, isoleucine, and leucine significantly (p < 0.05) decreased tail length of DNA (damaged portion) to 254.8 +/- 7.5, 235.6 +/- 5.6, and 271.5 +/- 19.9 mu m compared than positive control H2O2 (434.3 +/- 51.3 mu m). These results suggest that BCAAs can be used in the pharmaceutical or functional food industries as anti-inflammatory agents or anti-cancer agents.

• 出版日期2017-10