Analysis of resistance to powdery mildew in wheat based on expressed sequence tags (EST) technique

作者：Luo M; Kong XY; Jiang T; Jia C; Zhou RH; Jia JZ^*

来源：Acta Botanica Sinica, 2002, 44(5): 567-572.

• wheat
• Erysiphe graminis
• expressed sequence tags
• disease resistance gene

Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Erysiphe graminis DC. A conventional cDNA library was constructed, and a total of 1 500 clones picked randomly from the library were sequenced, three hundred and eighty-seven ESTs of them were unique, which got the Accession Number in GenBank. About 49.4% ESTs showed significant similarity to functions of known sequences in GenBank. There are 196 ESTs' with functions not able to be determined, and eighty-four ESTs were demonstrated to be. novel sequences. High-density dot membranes from unique clones were produced, and several disease resistance related genes were screened by differential hybridization.

• 出版日期2002-5
• 单位中国农业科学院