Molecular Typing of Enteroviruses, Adenoviruses, and Hepatitis A Viruses in Untreated and Treated Sewage of a Biological Treatment Plant in Greece

作者：Kokkinos P; Filippidou S; Karlou K; Vantarakis A^*

来源：Food and Environmental Virology, 2010, 2(2): 89-96.

DOI：10.1007/s12560-010-9036-3

• Wastewater
• Enterovirus
• Adenovirus
• Hepatitis A virus
• Virus detection

The effluents of a sewage treatment plant may contain infectious human viruses representing a major public health issue. In the present study, an 8 months survey was conducted in order to evaluate the presence of enteroviruses (EV), adenoviruses (AdV), and hepatitis A viruses (HAV) in untreated and treated sewage samples collected from a primary treatment municipal wastewater plant, located in the northeastern Greece. Reverse transcriptase-polymerase chain reaction (RT-PCR) and nested polymerase chain reaction techniques have been applied for viral nucleic acid detection. Positive samples were confirmed by sequencing, and comparative phylogenetic analysis was performed on the isolated viral strains. EVs, AdVs, and HAV have been detected in 40% (10/25), 40% (10/25), 4% (1/25) of the samples collected from the plant's inlet, and in 12% (3/25), 44% (11/25), 0% (0/25) of the samples collected from the plant's outlet. Adenovirus types 3 (Ad3), 10 (Ad10) and 41 (Ad41), and hepatitis A virus type H2 have been recognized, while for enteroviruses Coxsackie type A2 and Echovirus types 27 and 30 have been recorded. The results suggest that treated sewage may still contain human viruses and thereby represent a potential health hazard. Moreover, their possible reuse in agriculture or elsewhere must be considered with concern. Furthermore, this study shows the usefulness of molecular methods for virus detection, typing and virological quality analysis of sewage treatment plants.

• 出版日期2010-6