A new way of solid-phase microextraction fibers preparation for selected antibiotic drug determination by HPLC-MS

作者：Olszowy Pawel; Szultka Malgorzata; Nowaczyk Jacek; Buszewski Boguslaw^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2011, 879(25): 2542-2548.

DOI：10.1016/j.jchromb.2011.07.007

• Electropolymerisation
• Ozonization
• Solid phase microextraction
• Linezolid
• High performance liquid chromatography
• Mass spectrometry

The polypyrrole (PPy) and polythiophene (PTh) solid phase microextraction (SPME) coatings were obtained with the use of the electropolymerisation and linear sweep voltammetry. Such fibers were modified by an ozone treatment in a gaseous phase in the concentration of 2.1 +/- 10.2 x 10(-5) mol dm(-3). Both kinds of fibers were applied in the microextraction of linezolid from standard solutions to compare the extraction efficiencies displayed by these sorption phases. In these investigations a better adsorption capacity was obtained for polypyrrole fibers and hence only these kinds of fibers were utilized in the measurements from human plasma. In all measurements the concentrations of the drugs were in the range from 1 to 20 mu g ml(-1) (standard solutions) and 1 to 15 mu g ml(-1) (human plasma). Before the measurements, an optimization of the desorption solution experiments was performed. The correlation coefficients (R) obtained in the standard solution and human plasma were in the range from 0.8399 to 0.9970. The relative standard deviations (RSDs) were in the range of 0.1-7.6%.

• 出版日期2011-9-1