Alterations of pattern in immune response and vitellogenesis during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei

作者：Bae Sun Hye; Okutsu Tomoyuki; Kang Bong Jung; Wilder Marcy N^*

来源：Fisheries Science, 2013, 79(6): 895-903.

DOI：10.1007/s12562-013-0652-3

• Eyestalk ablation
• Immune response
• Litopenaeus vannamei
• Prophenoloxidase
• Total hemocyte count
• Vitellogenin

Eyestalk ablation is often used to induce ovarian development in female shrimp, but it possibly alters the animal's hormonal balance, and affects its physiology and immune system. Therefore, this study was conducted to investigate alterations to the immune response during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei. Sub-adult female shrimp could be induced to undergo ovarian development over a 20-day period by both unilateral and bilateral eyestalk ablation; however, ovaries did not reach the final mature stage in which cortical rods are observed. In terms of immune response, total hemocyte count decreased significantly at 4 h after unilateral ablation, and at 20 days after bilateral ablation. After 20 days, expression in the hemocytes of the immune-related genes prophenoloxidase and peroxinectin in bilaterally ablated shrimp increased significantly, whereas that for penaeidin, for lipopolysaccharide- and beta-glucan-binding protein, and for transglutaminase mRNA expression did not change significantly. The results of this study suggest that, while eyestalk ablation induces ovarian development, it may also bring about a hormonal imbalance that leads to immune-related genes being activated.

• 出版日期2013-11