Use of a small particle solid-core packing for improved efficiency and rapid measurement of sirolimus and everolimus by LC-MS/MS

作者：Morgan Phillip^*; Nwafor Magnus; Tredger Mike

来源：Biomedical Chromatography, 2016, 30(6): 983-985.

DOI：10.1002/bmc.3628

• sirolimus
• everolimus
• LC-MS
• MS
• solid-core
• Cortecs

Measurement of whole blood sirolimus and everolimus is required in order to optimize patient treatment following solid organ transplant. Assay by LC-MS/MS is increasingly preferred; however efficient use of the instrument and short turnaround times are crucial. Use of a 1.6 mu m solid-core packing HPLC column (Cortecs) gave significant increases in efficiency, sensitivity and throughput compared with an existing method, following simple protein precipitation of small-volume (20L) whole blood samples. Sirolimus, everolimus and the stable isotopic internal standard ((C2D4)-C-13 - everolimus) eluted at around 0.8min, and total analytical run time was 2.2min, saving almost 4min per sample compared with an existing method. Within-assay imprecision (CV) was 3.3-8.5%, and between-assay imprecision was 2.2-10.8%. Retrospective assay of external quality assurance samples and comparison of patient samples assayed in parallel showed only small differences (between +6.8 and -1.9%) in results using the Cortecs column when compared with the existing method. No significant interferences or ion suppression were observed.

• 出版日期2016-6