Antiproliferative effect of isolated isoquinoline alkaloid from Mucuna pruriens seeds in hepatic carcinoma cells

作者：Kumar Pranesh; Rawat Atul; Keshari Amit K; Singh Ashok K; Maity Siddhartha; De Arnab; Samanta Amalesh; Saha Sudipta^*

来源：Natural Product Research, 2016, 30(4): 460-463.

DOI：10.1080/14786419.2015.1020489

• isoquinoline alkaloid
• molecular docking
• Huh-7 cells
• caspase-3 and 8

The present study was undertaken to investigate the antiproliferative action of isolated M1 (6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) from Mucuna pruriens seeds using human hepatic carcinoma cell line (Huh-7 cells). Initially, docking studies was performed to find out the binding affinities of M1 to caspase-3 and 8 enzymes. Later, cytotoxic action of M1 was measured by cell growth inhibition (MTT), followed by caspase-3 and 8 enzymes assay colorimetrically. Our results collectively suggested that M1 had strong binding affinity to caspase-8 in molecular modelling. M1 possessed antiproliferative activity on Huh-7 cells (EC50 = 13.97 mu M) and also inhibited the action of caspase-8 enzyme, signified process of apoptosis. M1 was active against Huh-7 cells that may be useful for future hepatic cancer treatment.

• 出版日期2016
• 单位河北医科大学