Avoiding spots detection in analysis of electrophoretic gel images

作者：Daszykowski M; Bierczynska Krzysik A; Silberring J; Walczak B^*

来源：Chemometrics and Intelligent Laboratory Systems, 2010, 104(1): 2-7.

DOI：10.1016/j.chemolab.2009.10.001

• Image segmentation
• Significance analysis
• Discriminant PLS

Analysis of proteomic data, presented in form of 2D gel electropherograms, is still a real challenge and requires further improvements. Three main problems in data analysis are associated with (1) 'software-induced variance' (due to the different methods of data pre-processing or their different input parameters), (2) univariate significance analysis, and (3) requirements of spots detection and quantification. In our study, the advantages of multivariate significance analysis and a pixel-based approach are demonstrated upon the example of the real data set.

• 出版日期2010-11-15