Adjuvant arthritis influences expression of housekeeping genes

作者：Hanafy Sherif; Jamali Fakhreddin^*

来源：Inflammation Research, 2011, 60(6): 521-523.

DOI：10.1007/s00011-011-0327-4

• Polymerase chain reaction
• Adjuvant arthritis
• HK genes

Objective and method We determined whether adjuvant arthritis (AA) affects expression of three housekeeping (HK) genes, glyceraldehyde-3-phosphate-dehydrogenase (GAPDH), beta-actin and 18s ribosomal RNA (18s rRNA) in organs of Sprague-Dawley rats using the real-time reverse-transcription polymerase chain reaction (RT-PCR).
Results Expression of 18s rRNA was unaffected by AA whereas that of beta-actin was reduced by 52, 62 and 81% in the liver, kidney and intestine, respectively, but not in the heart. AA significantly reduced GAPDH expression in the intestine.
Conclusion For reliable results, the HK gene must be carefully selected.

• 出版日期2011-6