LINC01510 suppresses cell proliferation and invasion by inhibiting Wnt/β-catenin signaling in renal cell carcinoma

作者：Ma, Binbin; Zhang, Jianian; Zhou, Wenlong; Chu, Chenlong; Zhao, Chenhui; Zhang, Zhaohui; Huang, Tao^*

来源：Biochemical and Biophysical Research Communications, 2018, 505(1): 7-12.

DOI：10.1016/j.bbrc.2018.08.167

• LINC01510
• Cell proliferation
• Cell invasion
• Wnt/beta-catenin

The role of long non-coding RNA in Renal cell carcinoma (RCC) tumorigenesis and progression remains largely unknown. Here, we found that LINC01510 functions as a tumor suppressor in RCC tumorigenesis. We screened TCGA database and then found that LINC01510 is significantly down-regulated in malignant RCC tissues, and the lower expression of LINC01510 predicts poor prognosis. Moreover, the down regulated LINC01510 was further confirmed in our fresh tissues and cell lines. Biological functions assays shown that Ectopic expression of LINC01510 not only inhibits RCC cell proliferation both in vitro and in vivo, but also impairs cell invasion ability. Moreover, we found overexpression of LINC01510 inhibits the expression of CCND1 and CCNEI, as well as MMPs (MMP2, MMP7 and MMP9), and thus affecting RCC cell cycle and invasion. Meanwhile, Western blot assays revealed that the expression of beta-catenin is regulated by LINC01510; overexpression of beta-catenin could partly rescue the cell viability and invasion ability caused by ectopic expression of LINC01510. Taken together, we found that LINC01510 regulates cell proliferation and invasion by modulating Wnt/beta-catenin signaling in RCC.

• 出版日期2018-10-20
• 单位上海交通大学; 同济大学; 上海市闵行区中心医院