A novel measurement of allele discrimination for assessment of allele-specific silencing by RNA interference

作者：Takahashi Masaki; Hohjoh Hirohiko^*

来源：Molecular Biology Reports, 2014, 41(11): 7115-7120.

DOI：10.1007/s11033-014-3586-7

• RNA interference
• Small interfering RNA
• Allele-specific silencing
• Allele discrimination
• Novel formula

Allele-specific silencing by RNA interference (ASP-RNAi) is an atypical RNAi that is capable of discriminating target alleles from non-target alleles, and may be therapeutically useful for specific inhibition of disease-causing alleles without affecting their corresponding normal alleles. However, it is difficult to design and select small interfering RNA (siRNAs) that confer ASP-RNAi. A major problem is that there are few appropriate measures in determining optimal allele-specific siRNAs. Here we show two novel formulas for calculating a new measure of allele-discrimination, named "ASP-score''. The formulas and ASP-score allow for an unbiased determination of optimal siRNAs, and may contribute to characterizing such allele-specific siRNAs.

• 出版日期2014-11