Antinociceptive Activity of the Crude Methanolic Extract and Different Fractions of Viola serpens Wall

作者：Ghaffar Rukhsana^*; Saeed Muhammad; Ahmad Manzoor; Khan Haroon; Shehzad Omer; Naz Attiqa

来源：Journal of the Chemical Society of Pakistan, 2017, 39(4): 701-705.

• Viola serpens
• Antinociceptive
• Acetic-induced writhing test
• Formalin test
• Acute toxicity

The purpose of our study was to evaluate the toxicity and antinociceptive effect of the crude extract/fractions of Viola serpens in animal studies. The extract was found absolutely safe up to the doses of 2 g/kg in acute toxicity test. When studied in acetic acid induced writhing test, significant (p< 0.05) attenuation of painful sensation at doses, 100, 200 and 300 mg/kg (i.p.) was caused by the extract. Upon fractionation, hexane fraction was the most prominent fraction in pain reduction followed by chloroform and ethyl acetate fractions in a dose-dependent manner. Similarly, in formalin induced tail flick test, the extract antagonized the nociception in both phases, being more pronounced in late phase 100, 200 and 300 mg/kg (i.p). The n-hexane was the most active fraction in pain reduction followed by chloroform and ethyl acetate fractions. The crude extract and fractions of V. serpens possessed marked antinociceptive effects and seemed to be a safe drug that could provide a scientific rationale for its folk uses as analgesic.

• 出版日期2017-8