Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17 alpha/beta-exocyclic side chain stereochemistry

作者：Wu Tung Kung^*; Chang Yi Chun; Liu Yuan Ting; Chang Cheng Hsiang; Wen Hao Yu; Li Wen Hsuan; Shie Wen Shiang

来源：Organic and Biomolecular Chemistry, 2011, 9(4): 1092-1097.

DOI：10.1039/c0ob00582g

Site-saturated substitution in Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase at Ile705 position produced three chair-boat-chair (C-B-C) truncated tricyclic compounds, two 17 alpha-exocyclic protosteryl intermediates, two protosteryl C-17 truncated rearranged intermediates and the normal biosynthetic product, lanosterol. These results indicated the importance of the Ile705 residue in affecting lanosterol's C/D ring stabilization including 6-6-5 tricyclic and protosteryl C-17 cations and 17 alpha/beta-exocyclic side chain stereochemistry.

• 出版日期2011