Antiadenoviral effect of the alpha 5 beta 1 integrin receptor ligand, GRGDSP peptide, in serotypes that cause acute keratoconjunctivitis

作者：Uchio Eiichi^*; Kimura Ryoji; Huang Yuh Huey; Fuchigami Aya; Kadonosono Kazuaki; Hayashi Akio; Ishiko Hiroaki; Aoki Koki; Ohno Shigeaki

来源：Ophthalmologica, 2007, 221(5): 326-330.

DOI：10.1159/000104763

• adenovirus
• alpha 5 beta 1 integrin receptor ligand
• GRGDSP peptide
• conjunctivitis

Background/Aims: In adenoviral conjunctivitis, the infection process starts by the attachment of adenoviral fibers to conjunctival epithelial cells that contain the receptor for the adenovirus. The alpha 5 beta 1 integrin receptor ligand, GRGDSP peptide, contains the arginine-glycine-aspartate-binding motif which is common to the Coxsackie adenovirus receptor and integrins that are known to be adenoviral receptors. We evaluated the antiadenoviral effect of an expected adenoviral receptor inhibitor, GRGDSP peptide, in vitro. Methods: Adenovirus types 3, 4, 8, 19 and 37 were used. After calculating the 50% cytotoxic concentration of GRGDSP peptide, the adenovirus was cultivated with the agent for 7 days under serial dilution. Adenoviral DNA was qualitatively measured by real-time PCR. Results: GRGDSP peptide showed an inhibitory effect against adenoviral proliferation in all serotypes except type 4 in a dose-dependent manner. Conclusion: This result suggests that the alpha 5 beta 1 integrin receptor ligand, GRGDSP peptide, has antiadenoviral activity in vitro, and the possibility of being used for local treatment of epidemic keratoconjunctivitis.

• 出版日期2007