A propeptide-independent protease from Tannerella sp.6_1_58FAA_CT1 displays trypsin-like specificity

作者：Song, Qitao; Zhang, Xiaoyue; Li, Na; Shen, Jie; Cheng, Jiansong

来源：Journal of Basic Microbiology, 2017, 57(1): 50-56.

DOI：10.1002/jobm.201600486

• Protease
• Trypsin-like
• Propeptide
• Citrulline
• Tannerella

Despite the absence of any homologs of Tannerella forsythia KLIKK proteases in Tannerella sp.6_1_58FAA_CT1, the strain possesses a putative cysteine protease (G9S4N1) closely related to RgpB of Porphyromonas gingivalis. G9S4N1 lacks obvious propeptide that behaves as inhibitor of proteases and was proven to be a propeptide-independent protease. Unlike RgpB, which exclusively cleaves ArgXaa bonds, G9S4N1 exhibits both arginine- and citrulline-specific activities. Mutations of Asp177, a potential P1-Arg binding site, to uncharged or positively charged residues did not alter the substrate specificity of G9S4N1 significantly. Moreover, a group of arginine-specific proteases from different species including porcine trypsin, bovine thrombin, and a trypsin-like serine protease of dengue 2 virus CF40-Gly-NS3pro185 also display different specificity toward citrulline residue, suggesting that citrulline-modified protein might have different roles and destiny in biological processes involving various proteases.

• 出版日期2017-1
• 单位南开大学