Molecular identification and sex distribution of two chemosensory receptor families in Athetis lepigone by antennal transcriptome analysis

作者：Zhang, Ya-Nan^*; Ma, Ji-Fang; Sun, Liang; Dong, Zhi-Ping; Li, Zhao-Qun; Zhu, Xiu-Yun; Wang, Yi; Wang, Lu; Deng, Dao-Gui; Li, Jin-Bu^*

来源：Journal of Asia-Pacific Entomology, 2016, 19(3): 571-580.

DOI：10.1016/j.aspen.2016.05.009

• Antennal transcriptome
• Odorant receptor
• Ionotropic receptor
• Sex distribution

Chemosensory receptors play central roles in insect behavior and can potentially be used as new targets for pest control. Athetis lepigone Moschler (Lepidoptera: Noctuidae) is a polyphagous insect pest found in many countries in Europe and Asia. However, systemic identification of the chemosensory receptors in the antennae has not been reported. In the present study, we obtained the antennal transcriptome of A. lepigone using Illumina sequencing technology and identified 80 candidate chemosensory receptors, including 61 transcripts encoding for odorant receptors (ORs) and 19 for ionotropic receptors (IRs). This result is similar to that reported for other lepidopteran species, suggesting that our approach successfully identified ORs and IRs of A. lepigone. RT-PCR was used to determine the sex distribution patterns of all OR and IR genes. Some OR and IR genes showed male- or female-specific/predominant expression. Our results help understand the functions of chemosensory receptor genes in A. lepigone as well as other insects, and suggest that these genes could be potential targets for developing environment-friendly behavioral antagonists and pesticides against A. lepigone.

• 出版日期2016-9
• 单位中国农业科学院; 河北省农林科学院; 淮北师范大学