Diazaquinomycins E-G, Novel Diaza-Anthracene Analogs from a Marine-Derived Streptomyces sp.

作者：Mullowney Michael W; hAinmhire Eoghainin O; Shaikh Anam; Wei Xiaomei; Tanouye Urszula; Santarsiero Bernard D; Burdette Joanna E; Murphy Brian T^*

来源：Marine Drugs, 2014, 12(6): 3574-3586.

DOI：10.3390/md12063574

• actinomycete
• marine
• Streptomyces
• ovarian cancer
• OVCAR5
• diazaquinomycin

As part of our program to identify novel secondary metabolites that target drug-resistant ovarian cancers, a screening of our aquatic-derived actinomycete fraction library against a cisplatin-resistant ovarian cancer cell line (OVCAR5) led to the isolation of novel diaza-anthracene antibiotic diazaquinomycin E (DAQE; 1), the isomeric mixture of diazaquinomycin F (DAQF; 2) and diazaquinomycin G (DAQG; 3), and known analog diazaquinomycin A (DAQA; 4). The structures of DAQF and DAQG were solved through deconvolution of X-Ray diffraction data of their corresponding co-crystal. DAQE and DAQA exhibited moderate LC50 values against OVCAR5 of 9.0 and 8.8 mu M, respectively. At lethal concentrations of DAQA, evidence of DNA damage was observed via induction of apoptosis through cleaved-PARP. Herein, we will discuss the isolation, structure elucidation, and biological activity of these secondary metabolites.

• 出版日期2014-6