A crosstalk between chromatin remodeling and histone H3K4 methyltransferase complexes in endothelial cells regulates angiotensin II-induced cardiac hypertrophy

作者：Weng, Xinyu; Yu, Liming; Liang, Peng; Li, Luyang; Dai, Xin; Zhou, Bisheng; Wu, Xiaoyan; Xu, Huihui; Fang, Mingming^*; Chen, Qi; Xu, Yong

来源：Journal of Molecular and Cellular Cardiology, 2015, 82: 48-58.

DOI：10.1016/j.yjmcc.2015.02.010

• Vascular endothelial cell
• Cardiac hypertrophy
• Epigenetics
• Transcriptional regulation
• ET-1

Angiotensin II (Ang II) induces cardiac hypertrophy and fibrosis in part by stimulating endothelin (ET-1) transcription. The involvement of the epigenetic machinery in this process is largely undefined. In the present study, we examined the epigenetic maneuvering underlying cardiac hypertrophy and fibrosis following ET-1 transactivation by Ang II. In response to Ang II stimulation, core components of the mammalian chromatin remodeling complex (Brahma-related gene 1, or Brg1, and Brahma or Brm) and histone H3K4methylation complex (Ash2, absent, small, or homeotic discs 2, or Ash2 and WD domain repeat 5, or Wdr5) were recruited to the ET-1 promoter region in endothelial cells. Over-expression of Brg1/Brm or Ash2/Wdr5 enhanced while depletion of Brgl/Brm or Ash2/Wdr5 attenuated Ang II-induced ET-1 transactivation. Endothelial-specific knockdown of Brgl/Brm or Ash2/Wdr5 ameliorated cardiac hypertrophy both in vitro and in vivo. More important, Brg1/Brm interacted with Ash2/Wdr5 on the ET-1 promoter to catalyze H3K4 methylation. The crosstalk between Brg11/ Brm and Ash2/Wdr5 was mediated by myocardin-related transcription factor A (MRTF-A). In conclusion, our data have unveiled an epigenetic complex that links ET-1 transactivation in endothelial cells to Ang ll-induced cardiac hypertrophy and fibrosis.

• 出版日期2015-5
• 单位南京医科大学