Ultrapure chitosan oligomers as carriers for corneal gene transfer

作者：Klausner Eytan A^*; Zhang Zhong; Chapman Robert L; Multack Richard F; Volin Michael V

来源：Biomaterials, 2010, 31(7): 1814-1820.

DOI：10.1016/j.biomaterials.2009.10.031

• Ultrapure chitosan oligomers
• Chitosan-DNA nanoparticles
• Corneal gene therapy
• Non-viral gene transfer
• Cornea

NOVAFECT chitosans are ultrapure chitosan oligomers that were recently marketed as carriers for non-viral gene therapy. There are no reports on systematic design and improvement of formulations based on NOVAFECT chitosans for gene delivery. Therefore, we have designed and characterized chitosan-DNA nanoparticles based on NOVAFECT We found that the size of oligomeric chitosan-DNA nanoparticles is small, <= 98.2 +/- 4.4 nm. Zeta potential measurements of oligomeric chitosan-DNA nanoparticles exhibited a strong positive charge >= 44.1 +/- 3.5 millivolt. In vitro transfection studies demonstrated the ability of oligomeric chitosan-DNA nanoparticles to effectively transfect COS-7 cells. In rat corneas, injection of a select formulation of oligomeric chitosan-DNA nanoparticles into the stroma showed that (a) luciferase gene expression was 5.4 times greater than following administration of polyethylenimine-DNA nanoparticles; and (b) the cells that express the transgene, green fluorescent protein, were keratocytes (corneal fibroblasts). This study lays the foundation for evaluating oligomeric chitosan-DNA nanoparticles as pharmaceuticals for corneal gene therapy, a promising approach for the treatment of acquired and inherited corneal diseases that otherwise lead to blindness. Oligomeric chitosan-DNA nanoparticles can also be evaluated for the treatment of ocular diseases outside of the cornea, and for various additional gene therapy applications.

• 出版日期2010-3