A heterochromatin protein 1 (HP1) dimer and a proliferating cell nuclear antigen (PCNA) protein interact in vivo and are parts of a multiprotein complex involved in DNA replication and DNA repair

作者：Trembecka Lucas Dominika O; Dobrucki Jurek W^*

来源：Cell Cycle, 2012, 11(11): 2170-2175.

DOI：10.4161/cc.20673

• HP1 beta
• PCNA
• DNA replication
• DNA repair
• bimolecular fluorescence complementation analysis
• BiFC

Heterochromatin protein 1 (HP1) is a small non-histone chromosomal protein known as a dominant suppressor of position-effect variegation and a major component of heterochromatin. Posttranslationally modified HP1, through interaction with protein partners from different groups, can be involved in a number of nuclear processes, including gene activation, chromatin remodeling, replication and DNA repair. Using bimolecular fluorescence complementation assay and live cell imaging, we demonstrate that HP1 beta and PCNA, a key player in DNA replication, are closely spaced components of a multiprotein complex involved in replication, both in S phase and during DNA repair, and that the functional complex requires formation of an HP1 dimer.

• 出版日期2012-6-1