A C-di-GMP-proflavine-hemin supramolecular complex has peroxidase activity-implication for a simple colorimetric detection

作者：Nakayama Shizuka; Roelofs Kevin; Lee Vincent T; Sintim Herman O^*

来源：Molecular Biosystems, 2012, 8(3): 726-729.

DOI：10.1039/c2mb05430b

Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS(center dot+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.

• 出版日期2012