Process strategies for enhanced production of 1,3-propanediol by Lactobacillus reuteri using glycerol as a co-substrate

作者：Vieira P B^*; Kilikian B V; Bastos R V; Perpetuo E A; Nascimento C A O

来源：Biochemical Engineering Journal, 2015, 94: 30-38.

DOI：10.1016/j.bej.2014.11.003

• Lactobacillus reuteri
• Glycerol
• 1,3-Propanediol
• Batch process
• Bioreactor
• Microbial growth

1,3-Propanediol (PDO) is a bulk chemical used in the synthesis of polymers for terephthalates, cosmetics and lubricants, among other things. PDO can be produced by microorganisms growing anaerobically on glycerol. The objective of this study was to analyze the behavior of Lactobacillus reuteri ATCC23272 when cultivated in batch, repeated batch and continuous modes during the conversion of glycerol into 1,3-propanediol, with regards to the main parameters for viable industrial production: productivity (Q(PDO)), PDO concentration and yield on glycerol (Y-PDO/GLY). Cultures were performed in an MRS medium with glucose and glycerol as co-substrates, at 37 degrees C. Besides the bioreactor operation mode, the following features were evaluated: anaerobiosis, limited oxygen respiration, pH 5.5 and 6.2. In batch mode, the best condition was anaerobiosis at pH 5.5, which resulted in Y-PDO/GLY and Q(PDO) of 0.66 g(PDO) g(GLY)(-1) and 1.42 g L-1 h(-1), respectively. In repeated batch mode, the highest level of productivity was 4.12 g h(-1), when cells were first decanted and 80% of the liquid phase was replaced with fresh medium. In chemostat mode, Y-PDO/GLY of 0.70 g(PDO) g(GLY)(-1) was achieved and productivity in the steady state was 20% higher (4.92 g L-1 h(-1)), compared to the best result in the repeated batch mode. The highest PDO productivity, Q(PDO), in chemostat mode, was due to the highest rate of glucose consumption, which can be directly related to PDO productivity.

• 出版日期2015-2-15