Differentially regulated genes in Solanum tuberosum in response to "Mexican potato purple top phytoplasma" infection

作者：Maria Longoria Espinoza Rosa; Rubi Douriet Gamez Nadia; Lopez Meyer Melina; Quiroz Figueroa Francisco; Bueno Ibarra Mario; Mendez Lozano Jesus; Elena Santos Cervantes Maria; Felix Gastelum Ruben; Alicia Chavez Medina Jesus; Elena Leyva Lopez Norma

来源：Physiological and Molecular Plant Pathology, 2013, 81: 33-44.

DOI：10.1016/j.pmpp.2012.10.001

• Solanum tuberosum L.
• PPT disease
• Phytoplasma-plant interaction
• Differentially expressed genes
• SSH
• Macroarray screening
• Real-time PCR

Potato plants infected by phytoplasmas exhibit an array of symptoms that suggest interference within the normal balance of plant metabolism. Suppression subtractive hybridization (SSH), macroarray screening and real-time PCR were performed to identify differentially expressed genes in phytoplasma-infected in vitro potato plants. Gene expression analysis revealed the up-regulation of genes involved in several metabolic pathways, including ERF, WRKY, HSP90, SAP6, AN1-like ZFP, and an unknown protein; the down-regulation of genes encoding monodehydroascorbate reductase and a putative membrane protein was also observed. The results presented here provide the groundwork for addressing new questions to better understand the phytoplasma-plant interaction.

• 出版日期2013-1