Application of Dermal Microdialysis for the Determination of Bioavailability of Clobetasol Propionate Applied to the Skin of Human Subjects

作者：Au W L; Skinner M F; Benfeldt E; Verbeeck R K; Kanfer I^*

来源：Skin Pharmacology and Physiology, 2012, 25(1): 17-24.

DOI：10.1159/000330489

• Dermal microdialysis
• Clobetasol propionate
• Intralipid (R)
• Bioavailability
• Topical application

Dermal microdialysis was used to assess the bioavailability of a topical corticosteroid, clobetasol propionate, following application onto the skin of human subjects. The penetration of clobetasol propionate from a 4% m/v ethanolic solution applied onto 4 sites on one forearm of healthy human volunteers was studied. A lipid emulsion, Intralipid (R), was used as the perfusate and linear microdialysis probes with a 2-kDa cutoff were inserted intradermally at the designated sites. The results indicated that Intralipid could be used as a suitable perfusate for in vivo microdialysis of this lipophilic drug of interest. Furthermore, the study clearly demonstrated the application of dermal microdialysis as a valuable tool to assess the bioavailability/bioequivalence of clobetasol propionate penetration into the skin following topical application.

• 出版日期2012