Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1

作者：Lim Daina; Lu Yuning; Rudd Christopher E^*

来源：Immunology Letters, 2016, 172: 40-46.

DOI：10.1016/j.imlet.2016.02.004

• T-cells
• Talin
• LFA-1
• Adhesion

While the cytoskeletal protein talin binds to the beta-chain of LFA-1, the immune cell adaptor SKAP1 (SKAP-55) binds to the alpha-chain of the same integrin via RapL. Whereas calpain protease cleavage of talin is important for LFA-1 activation, it has been unclear whether SKAP1 can alter the function of talin or its associated adaptor RIAM in T-cells. In this paper, we report that Skap1-/- T-cells showed a reduction in the translocation of talin and RIAM to the contact interface of T-cells with antigenic beads or dendritic cells (DCs) presenting OVA peptide to OT-1 T-cells. In addition, Skap1-/- T-cells show an altered pattern of talin cleavage, while the expression of a cleavage resistant form of talin (L432G) restored the impaired adhesion of OT1 transgenic Skap1-/- T-cells with DCs. SKAP1 therefore can affect the function of talin in T-cells needed for optimal T-cell/DC conjugation.

• 出版日期2016-4
• 单位河北医科大学