A novel anti-MUC1 antibody against the MUC1 cytoplasmic tail domain: use in sensitive identification of poorly differentiated cells in adenocarcinoma of the stomach

作者：Yonezawa Suguru^*; Kitajima Shinichi; Higashi Michiyo; Osako Masahiko; Horinouchi Michiko; Yokoyama Seiya; Kitamoto Sho; Yamada Norishige; Tamura Yukihiro; Shimizu Takeshi; Tabata Mineo; Goto Masamichi

来源：Gastric Cancer, 2012, 15(4): 370-381.

DOI：10.1007/s10120-011-0125-2

• MUC1
• Cytoplasmic tail domain
• Monoclonal antibody
• Scirrhous gastric cancer
• Accurate detection

Isolated cancer cells of non-solid type poorly differentiated adenocarcinoma (por2) or signet-ring cell carcinoma (sig) are frequently seen in scirrhous gastric cancers with a very poor prognosis. These cells are often scattered in granulation tissue or desmoplastic fibrotic tissue and tend to be overlooked in routine pathological examination. We aimed to raise a novel antibody that can identify the isolated cancer cells easily. %26lt;br%26gt;Because the MUC1 cytoplasmic tail domain (CTD) has many biological roles including tumor progression and cell adhesion disturbance and is expected to be expressed in isolated cancer cells, we raised a novel monoclonal antibody (MAb) MUC1-014E against an intracellular nonrepeating 19-amino-acid sequence (RYVPPSSTDRSPYEKVSAG: N-1217-1235-C) of the MUC1 CTD, using a synthetic peptide including the 7-amino-acid epitope (STDRSPY: N-1223-1229-C). %26lt;br%26gt;In the immunohistochemical staining of 107 gastrectomy specimens including 48 por2 and 31 sig lesions, the MAb MUC1-014E showed high rates of positive staining (a parts per thousand yen5% of carcinoma cells stained) for por2 (100%) and sig (97%), and of the highest intensity staining (4+, a parts per thousand yen75% of carcinoma cells stained) for por2 (100%) and sig (90%). In the 89 biopsy specimens including 82 por2 and 38 sig lesions, the MAb MUC1-014E showed high rates of positive staining for por2 (100%) and sig (100%) and of 4+ staining for por2 (87%) and sig (84%). All the rates were significantly higher than those with cytokeratins (AE1/AE3 or CAM5.2). %26lt;br%26gt;The MAb MUC1-014E is very useful for accurate detection of isolated cancer cells in scirrhous gastric cancers.

• 出版日期2012-10