Hybrid mu CT-FMT imaging and image analysis

作者：Gremse Felix; Doleschel Dennis; Zafarnia Sara; Babler Anne; Jahnen Dechent Willi; Lammers Twan; Lederle Wiltrud; Kiessling Fabian^*

来源：Jove-Journal of Visualized Experiments, 2015, (100): e52770.

DOI：10.3791/52770

• Bioengineering
• Issue 100
• Fluorescence-mediated Tomography
• Computed Tomography
• Image Segmentation
• Multimodal Imaging
• Image Analysis
• Hybrid Imaging
• Biodistribution
• Diffuse Optical Tomography

Fluorescence-mediated tomography (FMT) enables longitudinal and quantitative determination of the fluorescence distribution in vivo and can be used to assess the biodistribution of novel probes and to assess disease progression using established molecular probes or reporter genes. The combination with an anatomical modality, e.g., micro computed tomography (mu CT), is beneficial for image analysis and for fluorescence reconstruction. We describe a protocol for multimodal mu CT-FMT imaging including the image processing steps necessary to extract quantitative measurements. After preparing the mice and performing the imaging, the multimodal data sets are registered. Subsequently, an improved fluorescence reconstruction is performed, which takes into account the shape of the mouse. For quantitative analysis, organ segmentations are generated based on the anatomical data using our interactive segmentation tool. Finally, the biodistribution curves are generated using a batch-processing feature. We show the applicability of the method by assessing the biodistribution of a well-known probe that binds to bones and joints.

• 出版日期2015-6