Novel Method for L-Methionine Production Catalyzed by the Aminotransferase ARO8 from Saccharomyces cerevisiae

作者：Wu, Yiping; Zha, Musu; Yin, Sheng^*; Yang, Huaqing; Boutet, Julien; Huet, Robert; Wang, Chengtao^*; Sun, Baoguo

来源：Journal of Agricultural and Food Chemistry, 2018, 66(24): 6116-6122.

DOI：10.1021/acs.jafc.8b01451

• L-methionine
• aminotransferase
• ARO8
• Saccharomyces cerevisiae

The aminotransferase ARO8 was proved to play an efficient role in conversion of L-methionine into methionol via the Ehrlich pathway in Saccharomyces cerevisiae in our previous work. In this work, the reversible transamination activity of ARO8 for conversion of alpha-keto-gamma-(methylthio) butyric acid (KMBA) into L-methionine was confirmed in vitro. ARO8 was cloned from S. cerevisiae S288c and overexpressed in Escherichia coli BL21. A 2-fold higher aminotransferase activity was detected in the recombinant strain ARO8-BL21, and ARO8 was detected in the supernatant of ARO8-BL21 lysate with IPTG induction by SDS-PAGE analysis. The recombinant ARO8 was then purified and used for transforming KMBA into L-methionine. An approximately 100% conversion rate of KMBA into L-methionine was achieved by optimized enzymatic reaction catalyzed by ARO8. This work fulfilled L-methionine biosynthesis catalyzed by the aminotransferase ARO8 using glutamate and KMBA, which provided a novel method for L-methionine production by enzymatic catalysis with the potential application prospect in industry.

• 出版日期2018-6-20
• 单位北京工商大学