Microflora of Mana, a nepalese rice koji

作者：Nikkuni S^*; Karki TB; Terao T; Suzuki C

来源：Journal of Fermentation and Bioengineering, 1996, 81(2): 168-170.

DOI：10.1016/0922-338X(96)87597-0

• Mana
• Murcha
• koji
• Nepal
• microflora
• Aspergillus oryzae

A rice koji Mana sample obtained in Nepal contained 1.5 x 10(6) cfu/g of Mucorales, 3.5 x 10(7) cfu/g of aspergilli and 1.1 x 10(5) cfu/g of lactic acid bacteria, while yeast was present at less than 10(3) cfu/g. All of the isolates of Aspergillus (5 strains) had metulae with length of 7-15 mu m and proved to be aflatoxin-negative. The sequence of partial 18S rRNA gene of the isolate was identical to those of Aspergillus oryzae and Aspergillus flavus. The isolates had the same restriction enzyme cleavage patterns of genomic DNA as that of A. oryzae. A. oryzae was found to be a dominant species in the Mana sample prepared with steamed rice.

• 出版日期1996