An In Vitro Evaluation of the Growth of Human Periodontal Ligament Fibroblasts after Exposure to a 4-META-containing Methacrylate-based Endodontic Sealer

作者：Morrison Bradley^*; Sidow Stephanie; McNally Kathleen; McPherson James; Chuang Augustine

来源：Journal of Endodontics, 2011, 37(6): 803-806.

DOI：10.1016/j.joen.2011.02.026

• Human periodontal ligament fibroblasts
• MetaSEAL
• methacrylate resin-based sealer

Introduction: In this study we evaluated the cytotoxic effects of MetaSEAL, a 4-META containing methacrylate-based endodontic sealer, on human periodontal ligament (HPDL) fibroblasts. There are a limited number of studies on the cytotoxic effects of MetaSEAL, and there are no studies on the cytotoxic effects of MetaSEAL on cells it might come into contact with in vivo. Methods: MetaSEAL concentrations of 25, 50, 100, 200, 400, and 800 mu g/mL were exposed to HPDL fibroblast cultures and evaluated at 1, 3, 7, 14, and 21 days. Controls included untreated cells and cells treated with ethanol, the vehicle for MetaSEAL suspension. Crystal violet staining in 24-well plates and the fluorescence-based CyQUANT Cell Proliferation Assay in 96-well plates assessed fibroblast viability. Results: Significant cytotoxicity against HPDL growth by MetaSEAL was both time- and concentration-dependent. At day 1 there were no significant cytotoxic effects, whereas by day 3, 800 mu g/mL concentration, by day 7, 200, 400, and 800 mu g/mL concentrations, and by day 14, 50, 100, 200, 400, and 800 mu g/mL concentrations were significantly cytotoxic. By day 21, all concentrations were significantly cytotoxic. These findings were confirmed by both the crystal violet and CyQUANT assays. Conclusions: MetaSEAL endodontic sealer has increasing HPDL cytotoxicity with both concentration and time exposure. (J Endod 2011;37:803-806)

• 出版日期2011-6