A New HMW-GS 1Bx23* Containing an Amino Acid Segment Similar to Collagen

作者：Wei, L.; Bai, S. G.; Hou, X. J.; Li, J. M.; Zhang, B.; Chen, W. J.; Liu, D. C.; Liu, B. L.^*; Zhang, H. G.

来源：Cereal Research Communications, 2014, 42(4): 658-667.

DOI：10.1556/CRC.2014.0012

• Tibetan wheat cultivar
• HMW-GS
• Glu-Bx
• allelic variation

Among 20 awnless Tibetan wheat cultivars analyzed by SDS-PAGE, the migration rate of an HMW-GS in XM001584 and XM001593, named 1Bx23*. was shown to be slightly faster than 1Bx6. and slower than Bx7. Its nucleotide sequence was isolated based on homology clones. In a phylogenetic tree of 1Bx genes, 1Bx23* was apparently clustered with 1Bx23. Compared with 1Bx23. eight single nucleotide replacements caused four single amino acid replacements in 1Bx23*. The deletion of "G" at base pair 1463 and insertion of "A" at 1509 bps induced a 42-nucleotide frame shift. "GQRQQAGQWQRPGQ" was replaced by "DKGNRQDNGNDRDK". The new segment cannot be found in other HMW-GSs, and it is very similar to a segment found in collagen. Moreover, an 18-nucleotide deletion made 1Bx23* six amino acids shorter than 1Bx23. The cultivar XM001593 had 28 chromosomes, which signifies that it was tetraploid wheat, and that the new HMW-GS1Bx23* cannot be used directly for breeding in common wheat.

• 出版日期2014-12
• 单位中国科学院西北高原生物研究所; 青海师范大学