Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

作者：Zhang Hongwei; Morrison Scott; Tang Yi Wei^*

来源：Clinics in Laboratory Medicine, 2015, 35(2): 461-+.

DOI：10.1016/j.cll.2015.02.006

• Gastroenteritis
• Multiplex PCR
• Laboratory-developed test

A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms are time-consuming and often lack sensitivity and specificity. Advances in molecular technology have provided new clinical diagnostic tools. Multiplex polymerase chain reaction (PCR)-based testing has been used in gastroenterology diagnostics in recent years. This article presents a review of recent laboratory-developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. It focuses on two commercial syndromic multiplex tests: Luminex xTAG Gastrointestinal Pathogen Panel and BioFire Film Array gastrointestinal test. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens.

• 出版日期2015-6