Acidosis Potentiates the Host Proinflammatory Interleukin-1 beta Response to Pseudomonas aeruginosa Infection

作者：Torres Iviana M; Patankar Yash R; Shabaneh Tamer B; Dolben Emily; Hogan Deborah A; Leib David A; Berwin Brent L^*

来源：Infection and Immunity, 2014, 82(11): 4689-4697.

DOI：10.1128/IAI.02024-14

Infection by Pseudomonas aeruginosa, and bacteria in general, frequently promotes acidification of the local microenvironment, and this is reinforced by pulmonary exertion and exacerbation. However, the consequence of an acidic environment on the host inflammatory response to P. aeruginosa infection is poorly understood. Here we report that the pivotal cellular and host proinflammatory interleukin-1 beta (IL-1 beta) response, which enables host clearance of the infection but can produce collateral inflammatory damage, is increased in response to P. aeruginosa infection within an acidic environment. Synergistic mechanisms that promote increased IL-1 beta release in response to P. aeruginosa infection in an acidic environment are increased pro-IL-1 beta induction and increased caspase-1 activity, the latter being dependent upon a functional type III secretion system of the bacteria and the NLRC4 inflammasome of the host. Using an in vivo peritonitis model, we have validated that the IL-1 beta inflammatory response is increased in mice in response to P. aeruginosa infection within an acidic microenvironment. These data reveal novel insights into the regulation and exacerbation of inflammatory responses to P. aeruginosa.

• 出版日期2014-11