Determination of phenolic acids and flavonoids in Rhinacanthus nasutus (L.) kurz by high-performance-liquid-chromatography with photodiode-array detection and tandem mass spectrometry

作者：Huang R T; Lu Y F; Inbaraj B Stephen; Chen B H^*

来源：Journal of Functional Foods, 2015, 12: 498-508.

DOI：10.1016/j.jff.2014.12.002

• Rhinacanthus nasutus
• Chinese herb
• Phenolic acids
• Flavonoids
• HPLC-PAD-MS/MS

The variety and content of phenolic acids and flavonoids in a Chinese herb Rhinacanthus nasutus were determined by developing an HPLC method coupled with photodiode-array-detection and tandem-mass-spectrometry. A high yield of phenolic acids and flavonoids was obtained using 30% ethanol-in-water and shaking in 60 degrees C water-bath for 3 h. By employing a C18 column and gradient mobile phase of 0.1% formic acid-in-water and acetonitrile, a total of 20 phenolic acids and 3 flavonoids were separated within 45 min with detection at 280 nm, flow rate at 0.8 mL/min and column temperature at 35 degrees C. Internal standards vanillic acid and quercetin were used for quantitation of phenolic acids and flavonoids in R. nasutus, respectively, which included caffeic acid derivatives (6.40 mg/g), quercetin derivatives (4.43 mg/g), ferulic acid derivatives (3.20 mg/g), p-coumaric acid derivatives (1.63 mg/g), sinapic acid hexoside (1.11 mg/g), kaempferol-3-O-rutinoside (1.02 mg/g), hydroxycinnamic acid derivatives (0.44 mg/g) and protocatechuic acid (0.39 mg/g).

• 出版日期2015-1