Magnesium incorporation into -TCP reduced its in vivo resorption by decreasing parathormone production

作者：Yassuda Debora H; Costa Neusa F M; Fernandes Gustavo O; Alves Gutemberg G; Granjeiro Jose M^*; Soares Gloria de A

来源：Journal of Biomedical Materials Research Part A, 2013, 101(7): 1986-1993.

DOI：10.1002/jbm.a.34502

• Beta-tricalcium phosphate
• -TCMP
• receptor activator of NF-kB ligand
• osteoprotegerin
• parathormone

Beta-tricalcium phosphate (-TCP), one of the most widely used bioresorbable materials for bone therapy, can be doped with magnesium ions, generating -TCMP. The objectives of this work were to evaluate, on a murine dental alveolus grafting model, the biocompatibility of -TCP and -TMCP granules by histomorphometric analysis, as well as the impact on plasmatic levels of receptor activator of nuclear factor B ligand (RANK-L), osteoprotegerin (OPG), osteocalcin, osteopontin, and parathormone (PTH) during bone repair, using Luminex multiplexing technology. After grafting for 42 days, -TCP grafted group presented higher bioresorption and induced more newly formed bone than -TCMP (p %26lt; 0.05). -TCP grafting also induced higher plasmatic levels of RANK-L, compared to -TCMP and control (blood clot) groups at 21st day (p %26lt; 0.05). PTH, which remained at low levels in control group, presented a time-dependent increase in grafted groups, attaining significantly higher levels with -TCP by the 42nd day (p %26lt; 0.05). RANK-L/OPG ratio increased on -TCP group and attained a peak on the 21st day. In conclusion, -TCP granules were more bioresorbable and osteogenic than -TCMP granules, and the resorption of both materials might have been affected by osteoclastogenesis modulated by changes in the plasmatic levels of PTH and RANK-L.

• 出版日期2013-7
• 单位中国计量科学研究院