A new family of peptide-nucleic acid nanostructures with potent transfection activities

作者：Bechinger Burkhard^*; Vidovic Verica; Bertani Philippe; Kichler Antoine

来源：Journal of Peptide Science, 2011, 17(2): 88-93.

DOI：10.1002/psc.1318

• solid-state NMR
• isothermal titration calorimetry
• siRNA
• DNA
• histidine-rich peptide
• antimicrobial peptide
• membrane interaction
• cell-penetrating peptide

A family of His-rich peptides has been shown to complex DNA and efficiently deliver these nucleic acids into eukaryotic cells. Therefore, these nanoscale complexes have potential applications in gene therapy. Here, we review a number of spectroscopic and biophysical investigations aimed at characterizing the supramolecular interactions of the peptides with the nucleic acids and when overcoming the membrane barriers of the cell. Furthermore, solid-state NMR distance measurements for the first time reveal close interatomic distances between the amino acid side chains and the DNA phosphates within the transfection complex. A recent study indicates that the peptides are also potent transfectants of siRNAs and they could thereby be of potential interest for gene silencing therapies using these compounds.

• 出版日期2011-2