Development of candidate combination vaccine for hepatitis E and hepatitis B: A liposome encapsulation approach

作者：Shrivastava Shubham; Lole Kavita S; Tripathy Anuradha S; Shaligram Umesh S; Arankalle Vidya A^*

来源：Vaccine, 2009, 27(47): 6582-6588.

DOI：10.1016/j.vaccine.2009.08.033

• Combination vaccine
• Liposome
• Hepatitis E
• Hepatitis B
• Co-delivery approach

To reduce extra injections, cost and ensure better coverage, use of combination vaccines is preferable. An attempt was made to evaluate the encapsulation of hepatitis E virus neutralizing epitope (NE) region and hepatitis B virus surface antigen (HBsAg) in liposomes as DNAs, proteins and DNA + protein. Mice groups were immunized with different liposome-encapsulated formulations and monitored for anti-HEV and anti-HBs titres. IgG subtypes, antigen-specific lymphocyte proliferation and cytokine levels. The protective levels of anti-HBs and in vitro virus-binding capacity of anti-HEV antibodies were assessed. Liposome-encapsulated DNA either singly or in combination did not elicit antibody response. Anti-HEV and anti-HBs IgG titres of individual component of protein alone (Lipo-E-P/Lipo-B-P) or DNA + protein formulations (Lipo-E-DP/Lipo-B-DP) were comparable to respective titres in combination vaccine of protein (Lipo-BE-P) and DNA + protein formulations (Lipo-BE-DP). IgG1 levels were significantly higher in Lipo-BE-P group whereas, equivalent levels of IgG1 and IgG2a were observed in Lipo-BE-DP group against both components of the vaccine. Combination vaccine group showed mixed Th1/Th2 cytokine profile. Liposome entrapped NE and HBsAg in protein and DNA + protein formats induce excellent immune response to both the components and need to be evaluated in higher animals.

• 出版日期2009-11-5