NF-kappa B-Dependent Upregulation of NCX1 Induced by Angiotensin II Contributes to Calcium Influx in Rat Aortic Smooth Muscle Cells

作者：Liu, Bei; Yang, Lixia; Zhang, Bin; Kuang, Chenwei; Huang, Shiliang; Guo, Ruiwei^*

来源：Canadian Journal of Cardiology, 2016, 32(11): 1356.e11.

DOI：10.1016/j.cjca.2016.02.060

• NA+/CA2+ EXCHANGER
• PARAVENTRICULAR NUCLEUS
• INDUCED-HYPERTENSION
• GENE-EXPRESSION
• TYPE-1 RECEPTOR
• BLOOD-PRESSURE
• CA2+ ENTRY
• HOMEOSTASIS
• CHANNELS
• PROLIFERATION

Background: The reverse mode of Na+/Ca2+ exchanger (NCX) 1 can transport Ca2+ into cells and is involved in the contractile regulation of vascular smooth muscle cells (VSMCs) and the development of hypertension. We hypothesized that upregulation of NCX1 expression induced by angiotensin II (Ang II) could be dependent on activation of lnuclear factorekappa B (NF-kappa B) and contributes to Ca2+ influx in VSMCs.
Methods: An osmotic minipump was implanted for administration of Ang II in Sprague-Dawley rats, and blood pressure, as well as NCX1 expression, in the aorta was measured. VSMCs were cultured to verify that Ang II-upregulated NCX1 expression is dependent on activation of NF-kappa B and contributes to Ca2+ influx.
Results: Ang II-upregulated NCX1 expression in rat aortas (2.1-fold at day 6) and VSMCs (1.7-fold at 24 hours) and NF-kappa B knockdown and p38 mitogen-activated protein kinase (MAPK) inhibitor resulted in 2.1-and 2.0-fold decreases in Ang II-upregulated NCX1 expression in VSMCs. KB-R7943 (an inhibitor of NCX1 reversal) and NCX1 knockdown decreased Ang II-induced Ca2+ influx 1.4-and 1.3-fold, respectively. KB-R7943 and removal of extracellular Na+ decreased Ang II-initiated store depletionemediated Ca2+ entry by 1.5-and 1.3-fold, respectively. Moreover, NF-kappa B knockdown and use of a p38 MAPK inhibitor resulted in about 1.3-fold decreases in Ang IIeinduced Ca2+ influx through activation of reverse-mode NCX1.
Conclusions: Ang II upregulates NCX1 expression through p38 MAPK and NF-kB pathways, and reverse-mode NCX1 plays an important part in Ang II-induced Ca2+ influx in VSMCs, which may be associated with Ang IIeinitiated store-operated channel entry.

• 出版日期2016-11
• 单位成都军区昆明总医院