Aqueous Extract of Crataegus azarolus Protects Against DNA Damage in Human Lymphoblast Cell K562 and Enhances Antioxidant Activity

作者：Mustapha Nadia; Bouhlel Ines; Chaabane Fadwa; Bzeouich Imen Mokdad; Ghedira Kamel; Hennebelle Thierry; Chekir Ghedira Leila^*

来源：Applied Biochemistry and Biotechnology, 2014, 172(4): 2266-2275.

DOI：10.1007/s12010-013-0667-3

• Crataegus azarolus
• Antigenotoxic activity
• Comet assay
• Cellular antioxidant activity
• K562

The present study was carried out to characterize the cellular antioxidant effect of the aqueous extract of Crataegus azarolus and its antigenotoxic potential using human myelogenous cells, K562. The antioxidant capacity of this extract was evaluated by determining its cellular antioxidant activity (CAA) in K562 cells. Also, preceding antigenotoxicity assessment, its eventual genotoxicity property was investigated by evaluating its capacity to induce the DNA degradation of treated cell nuclei. As no genotoxicity was detected at different exposure times, its ability to protect cell DNA against H2O2 oxidative effect was investigated, using the %26quot;comet assay.%26quot; It appears that 800 mu g/mL of extract inhibited the genotoxicity induced by H2O2 with a rate of 41.30 %, after 4 h of incubation. In addition, this extract revealed a significant cellular antioxidant capacity against the reactive oxygen species in K562 cells.

• 出版日期2014-2