Antioxidant and antiangiogenic activity of Astronium graveolens Jacq. leaves

作者：Hernandez Vanessa; Malafronte Nicola; Mora Flor; Pesca Maria S; Aquino Rita P; Mencherini Teresa^*

来源：Natural Product Research, 2014, 28(12): 917-922.

DOI：10.1080/14786419.2014.889134

• Astronium graveolens
• antioxidant and antiangiogenic activity
• placental growth factor
• 1
• 2
• 3
• 4
• 6-penta-O-galloyl-d-glucopyranose

Angiogenesis is involved in many physiological and pathological conditions. Natural compounds with antioxidant activity have also been reported to possess potent antiangiogenic properties by regulating angiogenesis modulators such as vascular endothelial growth factor (VEGF). Based on this, we screened the antioxidant and antiangiogenic activities of Astronium graveolens leaf extracts by a DPPH test and a competitive enzyme-linked immunosorbent assay, respectively. MeOH extract expressed a significant free radical-scavenging activity (EC50=37.65g/mL) and it was able to inhibit the interaction between placental growth factor (PlGF) (placental growth factor), a VEGF family member, and its receptor Flt-1 by more than 50% at 1mg/mL. 1,2,3,4,6-Penta-O-galloyl-d-glucopyranose, 6 is the most active compound of the extract. It exhibited a high potency in scavenging DPPH (EC50=2.16g/mL) and reduced by 58% the PlGF/Flt-1 interaction at a concentration of 50M. Moreover, the known compounds (1-6) have been isolated for the first time in A. graveolens.

• 出版日期2014-6-18