Development of nuclear microsatellite markers for the critically endangered freshwater macrophyte, Nuphar submersa (Nymphaeaceae), and cross-species amplification in six additional Nuphar taxa

作者：Yokogawa Masashi^*; Shiga Takashi; Kaneko Shingo; Isagi Yuji

来源：Conservation Genetics Resources, 2012, 4(2): 295-298.

DOI：10.1007/s12686-011-9528-y

• Conservation genetics
• Endangered species
• Microsatellite
• Nymphaeaceae
• Nuphar submersa

We developed and characterized 13 microsatellite markers for Nuphar submersa, a critically endangered freshwater macrophyte of central Japan. In all the studied populations, ten out of isolated 13 loci showed polymorphism. The 13 loci showed one to five alleles per locus, with observed and expected heterozygosities ranging from 0 to 0.40 and from 0 to 0.67, respectively. Between 5 and 11 of the 13 loci were successfully amplified across the six other Nuphar taxa. The markers described here will be useful for a conservation genetic study of the species and informing the development of effective conservation programs of N. submersa and related species.

• 出版日期2012-6