Development of an antibody-based diagnostic method for the identification of Bemisia tabaci biotype B

作者：Baek Ji Hyeong; Lee Hye Jung; Kim Young Ho; Lim Kook Jin; Lee Si Hyeock; Kim Bum Joon^*

来源：Pesticide Biochemistry and Physiology, 2016, 131: 18-23.

DOI：10.1016/j.pestbp.2016.02.003

• Bemisia tabaci
• Whitefly
• Biotype
• Protein biomarker
• Carboxylesterase

The whitefly Bemisia tabaci is a very destructive pest. B. tabaci is composed of various morphologically undistinguishable biotypes, among which biotypes B and Q, in particular, draw attention because of their wide distribution in Korea and differential potentials for insecticide resistance development. To develop a biotypespecific protein marker that can readily distinguishes biotypes B from other biotypes in the field, we established an ELISA protocol based on carboxylesterase 2 (COE2), which is more abundantly expressed in biotypes B compared with Q. Recombinant COE2 was expressed, purified and used for antibody construction. Polydonal antibodies specific to B. tabaci COE2 [anti-COE2 pAb and deglycosylated anti-COE2 pAb (DG anti-COE2 pAb)] revealed a 3-9-fold higher reactivity to biotype B COE2 than biotype Q COE2 by Western blot and ELISA analyses. DG anti-COE2 pAb exhibited low non-specific activity, demonstrating its compatibility in diagnosing biotypes. Western blot and ELISA analyses determined that one of the 11 field populations examined was biotype B and the others were biotype Q suggesting the saturation of biotype Q in Korea. DG anti-COE2 pAb discriminates B. tabaci biotypes B and Q with high specificity and accuracy and could be useful for the development of a B. tabaci biotype diagnosis kit for on-site field applications.

• 出版日期2016-7