Analysis of microRNA and mRNA expression profiles highlights alterations in modulation of the MAPK pathway under octanal exposure

作者：Song Mi Kyung; Choi Han Seam; Lee Hyo Sun; Kim Youn Jung; Park Yong Keun; Ryu Jae Chun^*

来源：Environmental Toxicology and Pharmacology, 2014, 37(1): 84-94.

DOI：10.1016/j.etap.2013.11.005

• MicroRNA
• Target mRNAs
• Aldehydes
• Octanal
• Mitogen-activated protein kinase
• Microarray

Previous environmental microRNA (miRNA) studies have investigated a limited number of candidate miRNAs and have not evaluated functional effects on gene expression. In this study, we aimed to identify octanal (OC)-sensitive miRNAs and to characterize the relationships between miRNAs and expression of candidate genes involved in OC-induced toxicity.
Microarray analysis identified 15 miRNAs that were differentially expressed in OC-exposed A549 human alveolar cells. Integrated analyses of miRNA and mRNA expression profiles identified significant miRNA-mRNA anti-correlations. GO analysis of 101 putative target genes showed that the biological category 'MAPK signaling pathway' was prominently annotated. Moreover, we detected increased phosphorylation of p38 MAPK in the OC-exposed group.
By integrating the transcriptome and microRNAome, we provide evidence that OC can affect MAPK-induced toxicity signaling. Therefore, this study demon

• 出版日期2014-1